اثر ازن‌درمانی و تمرین هوازی با شدت متوسط بر مقدار پروتئین‌های شلترین قلب موش‌های مبتلا به استئوآریت زانو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی،، پردیس بین‌المللی کیش، دانشگاه تهران، کیش، ایران

2 استاد گروه فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

3 استادیار گروه فیزیولوژی ورزشی، پردیس بین‌المللی کیش، دانشگاه تهران، کیش، ایران

چکیده

 
استئوآرتریت (OA) از بیماری‌های شایع در جهان است که به‌‌دلیل کاهش فعالیت بدنی، احتمال بروز بیماری‌های قلبی عروقی بیماران را افزایش می‌دهد. هدف پژوهش حاضر، مقایسۀ تمرین هوازی و ازن درمانی بر تغییرات پروتئین‌های شلترین بافت قلب موش‌های مبتلا به OA است. این تحقیق از نوع توسعه‌ای و روش آن تجربی بود که در آن 15 سر موش نر 8 هفته‌ای با میانگین وزنی 15±235 گرم، پس از القای مدل OA، تصادفی به سه گروه 5 تایی بیمار (OA)، بیمار و ازن‌درمانی (OAO) (ازن با غلظت 20 μg/ml، یک بار در هفته در خط مفصلی تیبیوفمورال 3 مرتبه تزریق شد) و بیمار و تمرین هوازی (OAE) (3 جلسه تمرین در هفته با سرعت 16 متر در دقیقه به‌صورت تداومی 8 هفته انجام گرفت) تقسیم شدند. 48 ساعت پس از پایان تمرین، بافت قلب استخراج و با روش وسترن بلات بیان پروتئین‌های RAP1،TIN2 و  TTP1قلب اندازه‌گیری شد. داده‌ها با استفاده از آزمون تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنا‌داری کوچک‌تر از 05/0 بررسی شد.
نتایج نشان داد گروهOAE  بیشترین افزایش مقدار پروتئین‌های TPP1 را در مقایسه با گروه‌های (P=0.016) OA وP=0.001)OAO) دارد، ولی تفاوت معناداری بین دو گروه OAO و P=0.319) OA ) مشاهده نشد. در سه گروه نیز تغییرات معناداری در مقدار پروتئین RAP1 (P=0.051)  مشاهده نشد. همچنین افزایش مقدار پروتئین TIN2 در گروه OAE در مقایسه با گروه (P=0.029) OA معنادار گزارش شد، اما تفاوت معناداری بین گروه OA و (P=0.277) OAO و OAO و OAE (P=0.219)  وجود نداشت.
در مجموع، نتایج نشان داد 8 هفته تمرین هوازی با شدت متوسط سبب افزایش مقدار پروتئین‌های TPP1، TIN2 و RAP1 بافت قلب موش‌هایOA  می‌شود. اما در خصوص آثار ازن‌درمانی بر تغییرات پروتئین‌های شلترین بافت قلبی، به تحقیقات بیشتری نیاز است.

کلیدواژه‌ها


عنوان مقاله [English]

Comparing The Effect of Moderate-intensity Aerobic Training and Ozone Therapy on Shelterin Expression Proteins in Heart Osteoarthritis Rats

نویسندگان [English]

  • GholamReza Karimi 1
  • Abbas ali Gaeini 2
  • Reza Nouri 3
  • Azadeh Gilani 1
1 Ph.D Candidate, Department of Exercise Physiology, University of Tehran, Kish International Campus, Kish, Iran
2 Professor of Exercise Physiology, Faculty of Physical Education and Exercise Sciences, University of Tehran, Iran
3 Assistant Professor of Exercise Physiology, University of Tehran, Kish International Campus, Tehran, Iran
چکیده [English]

 
Osteoarthritis (OA) is the most common disease of joints caused by degradation of articular cartilage and subchondral bone. It is unknown whether different therapy exert differential cellular effects. On the other hand, Since OA may be associated with CVD and decrease Telomeres length. Telomeres protect chromosome ends and Integral for the maintenance of telomeres is the protein complex Shelterin. In this study, the effects of ozone (O3) and aerobic training were considered on Shelterin expression proteins in heart rats with knee OA. In this experimental study, knee OA was induced by surgical method in rats. Fifteen male Wistar rats were randomly divided into three groups: knee OA control group (OA); OA plus exercise group (OAE) and OA plus O3 group (OAO). The aerobic training (treadmill; 16 m/min;50 min/day) started 4 weeks after the surgery, 3 days/week for 8 weeks. Rats in the OAO group received O3 at the concentration of 20μg/ml, once weekly for 3 weeks. 48 hours after the programs, cartilage and heart tissues were isolated and expression of RAP1, TIN2 and TPP1 were considered using Western Blot. In OAE significantly increased the expression of RAP1 compared to the OA and OAO groups. But not difference between OA and OAO groups. In OAE group had significantly higher expression of TIN2 compared to the OAO and OAE, and no difference between OAE and OAO groups. Also, there was no significant difference in the expression of TPP1 and between three groups. Aerobic Exercise improved cardiac Shelterin proteins expression in OA rats, rather than the Ozone therapy groups.

کلیدواژه‌ها [English]

  • erobic exercise
  • osteoarthritis
  • Ozone
  • RAP1
  • TIN2
  • TPP1
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