تأثیر استفادة همزمان از عصارة هستة انگور پوشش داده‌شده با نانوذرة کیتوزان و یک دوره تمرین تناوبی بر میزان آپوپتوز کاردیومیوسیت‌های مدل انفاراکتوس قلبی در موش صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

2 استادیار، گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

3 دانشیار،گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان (خوراسگان)، اصفهان، ایران

4 مرکز تحقیقات فن‌آوران بافت و ژن پا سارگارد، شرکت هیستولوژیک، تهران، ایران

چکیده

 

ایسکمی موجب افزایش تولید گونه‌های واکنش‌پذیر اکسیژن و تنش اکسایشی می‌شود که به‌طور مستقیم، با اثر بر غشا و پروتئین‌ها یا غیرمستقیم با تحریک مسیرهای آپوپتوز به آسیب سلولی منجر می‌شود. یکی از گیاهان دارای خاصیت ضداکسایشی عصارة هستة انگور است که می‌تواند تولید رادیکال‌هایی آزاد را کاهش دهد. هدف از این پژوهش بررسی تأثیر استفادة همزمان از عصارة هستة انگور پوشش داده‌شده با نانوذرة کیتوزان و یک دوره تمرین تناوبی بر میزان آپوپتوز کاردیومیوسیت‌های مدل انفاراکتوس قلبی در موش صحرایی بود. در این مطالعة تجربی 25 سر رت نر نژاد ویستار که از طریق القای ایسکمی قلبی با تزریق زیرجلدی ایزوپروترنول (85 mg/kg) دچار سکتة قلبی شده بودند، انتخاب شدند. رت‌های این مطالعه به‌طور تصادفی در 5 گروه شامل گروه کنترل سالم، گروه کنترل سکتة قلبی، گروه سکتة قلبی+هستة انگور، گروه سکته +تمرین تناوبی هوازی و گروه سکتة قلبی+هسته انگور+ تمرین تناوبی هوازی قرار گرفتند. تمرین تناوبی هوازی با 7 تناوب اینتروال هر تناوب شامل 4 دقیقه با شدت 80 تا 90 %Vo2max و 3 دقیقه با شدت 65 تا 75 %Vo2maxانجام گرفت. داده‌ها با استفاده از آزمون‌های آماری شاپیرو ویلک، تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح 05/0≥ α تحلیل شد. نتایج نشان داد Casp9 و Casp3 در گروه القای سکتة قلبی افزایش معنا‌داری نسبت به گروه کنترل سالم دارد (001/0 P=). در مقایسه با گروه سکتة قلبی تنها گروه سکته + تمرین+ هستة انگور کاهش معنا‌داری را درCasp3  و Casps9 و افزایش معنا‌داری را در مقادیر SOD نشان داد (001/0P=). بنابراین به‌نظر می‌رسد تمرین تناوبی هوازی و عصارة انگور با تنظیم منفی فاکتور التهابی Casp3 و Casps9 بافت قلب می‌تواند موجب مهار اپوپتوز سلول‌های قلبی پس از وقوع سکتة قلبی و همچنین کاهش آسیب بافت قلبی شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Concomitant Use of Grape Seed Extract Coated with Chitosan Nanoparticles and One Period of HIIT on The Rate of Cardiomyocyte Apoptosis of The Myocardial Infarction Model in Rats

نویسندگان [English]

  • Hamid Mohammadi Hosein Abady 1
  • Kkhosro Jalali 2
  • Gholamreza Sharifi 3
  • Zohreh Mazaheri tirani 4
1 PhD student, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University,Isfahan, Iran
2 Department of Exercise Physiology, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran
3 Associate Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
4 Basic Medical Science Research Center, Histogenotech Company, Tehran, Iran
چکیده [English]

 

Ischemia increases the production of reactive oxygen species and oxidative stress, which leads to cell damage directly by acting on membranes and proteins or indirectly by stimulating apoptotic pathways. One of the plants has antioxidant properties of grape seed extract that can reduce the production of free radicals. The aim of this study was to investigate the effect of concomitant use of grape seed extract coated with chitosan nanoparticles and one period of HIIT on the rate of cardiomyocyte apoptosis of the myocardial infarction model in rats. In this experimental study, 25 male Wistar rats who developed myocardial infarction by subcutaneous injection of isoprenaline(85mg/kg) were selected, Rats in this study were randomly divided into 5 groups:Control, Strok, Strok+Nano-Sup, Strok+AIT and Strok+Nano-Sup+AIT. AIT was performed with 7 intervals of each interval, including 4 minutes with intensity of 80 to 90% Vo2max and 3 minutes with intensity of 65 to 75% Vo2max. Data were analyzed using Shapiro-Wilk statistical tests, one-way analysis of variance and tokey post hoc test at α =0/05. The results showed that Casp9 and Casp3 in the myocardial induction group had a significant increase compared to the healthy control group (P = 0.001). compared with the myocardial infarction group, only the stroke + exercise + grape seed group showed a significant decrease in Casp3 and Casps9 and a significant increase in SOD values (P = 0.001). Therefore, it seems that HIIT and grape extract negatively regulate the inflammatory factor Casp3 and Casps9 of heart tissue can inhibit cardiac cell apoptosis after myocardial infarction and also reduce heart tissue damage.

کلیدواژه‌ها [English]

  • Casp3
  • Casps9
  • grape seed extract
  • HIIT
  • Myocardial Infarction

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پژوهش های فیزیولوژی و مدیریت در ورزش
دوره 13، شماره 3
آذر 1400
صفحه 55-69

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