عنوان مقاله [English]
The aim of this study was to compare the response of some salivary inflammatory markers including interferon gamma (IFN-g) and interleukin-6 (IL-6) in men with different ages following an exhaustive exercise with hand and leg ergometer. 36 healthy men in three age groups of children (12.7± 1.7 yr), middle-aged (34.3± 4.9 yr) and elderly (55±2.1 yr) performed two types of leg and arm ergometer protocols to exhaustion. Initial intensity of leg ergometer was 50 watt and arm ergometer was 25 watt. 25 watts were added to both protocols every 2 minutes. Salivary markers were measured before and immediately after both protocols. The data were analyzed by dependent t test, one-way analysis of variance and Tukey post hoc test. Both protocols significantly increased the values of the IFN-g and IL-6 in all three groups, but no significant differences were observed in the values of these markers in arm and leg exercises among the groups. When groups were compared in arm exercises, the amounts of IFN-g in middle-aged and elderly groups showed a greater increase than children group, but there was no significant difference in values of IL-6 among the groups. Also, while comparing the groups in the leg exercises, no significant differences were observed in levels of IFN-g and IL-6. Given these results, it can be noted that increased salivary cytokines of IL-6 and IFN-g following exhaustive exercise, particularly in the elderly and children groups, may be a defensive mechanism against exercise-induced stress.
1.Ahmadian M, Dabidi Roshan V and Dabirian M. 2014. Effect of Arm and Leg Exercise on Heart Autonomic Function in Children. International Journal of Sport Studies. Vol., 4 (7), 799-805.
2.Aps J. K. M and L. C. Martens. 2005. Review: the physiology of saliva and transfer of drugs into saliva. Forensic Science International, vol. 150, no. 2-3, pp. 119–131.
3.Baum M1, Müller-Steinhardt M, Liesen H, Kirchner H. 1997. Moderate and exhaustive endurance exercise influences the interferon-gamma levels in whole-blood culture supernatants. Eur J Appl Physiol Occup Physiol, 76(2):165-9.
4.Bean JF, Vora A, Frontera WR. 2004. Benefits of exercise for community dwelling older adults. Arch Phys Med Rehab, 85: 31–42.
5.Cengiz A. 2005. Relationship between the 30-second Wingate test and characteristics of isometric and explosive leg strength in young subjects. Journal of Strength and Conditioning Research, 19(3), 658–666.
6.Chiappin S, G. Antonelli, R. Gatti, and E. F. De Palo. 2007. Saliva specimen: a new laboratory tool for diagnostic and basic investigation. Clinica Chimica Acta, vol. 383, no. 1-2, pp. 30–40.
7.DeRijk RH, Boelen A, Tilders FJ, Berkenbosch F. 1994. Induction of plasma interleukin-6 by circulating adrenaline in the rat. Psychoneuroendocrinology, 19: 155–63.
8.Emilson C, Ronaldo V, Maria A. 2003. Oxygen uptake during Wingate tests for arms and legs in swimmers and water polo players. Rev Bras Med Esporte _ Vol. 9, N. 3 pp: 141-144.
9.Eriksson BO and Saltin B. 1974. Muscle metabolism during exercise in boys aged 11 to 16 years compared to adults. Acta Paediatr Belg 28: 257-265.
10.Falsey AR, Walsh EE. 2005. Respiratory syncytial virus infection in elderly adults. Drugs Aging, 22:577–87.
11.Fernandez-Botran R, Miller J. J, Burns V. E and newton T. L (2011) Correlations among inflammatory markers in plasma, saliva and oral mucosal transudate in post-menopausal women with past intimate partner violence. Brain, Behavior, and Immunity. 25 (2): 314-321.
12.Fischer CP. 2006. Interleukin-6 in acute exercise and training: what is the biological relevance? Exerc Immunol Rev, 12:6–33.
13.Flaishon L, Topilski I, Shoseyov D, Hershkoviz R, Fireman E, Levo Y, et al. 2002. Cutting edge: anti-inflammatory properties of low levels of IFN-gamma. J Immunol, 168(8):3707–11.
14.Gleeson, M. 2006. Immune system adaptation in elite athletes, Current Opinion in Clinical Nutrition and Metabolic Care, vol. 9, no. 6, pp. 659–665.
15. Heesen C, Gold SM, Hartmann S, Mladek M, Reer R, Braumann KM, Wiedemann K, Schulz KH. 2003. Endocrine and cytokine responses to standardized physical stress in multiple sclerosis. Brain Behav Immun 17: 473–481.
16.Helge, J.W et al. 2011. IL-6 release is higher across arm than leg muscles during whole body exercise. Exp Physiol. Jun;96(6):590-8.
17.Hovanloo et al 2013. Comparison the cardiovascular, metabolical and hematological responses to two type of upper and lower body exercises. International Journal of Sport Studies. Vol., 3 (12), 1380-1386.
18.Jafari A et al. 2013. Comparison of Exercise-Induced Inflammatory Response in Serum and Saliva of Healthy Men. Journal of Experimental Animal Biology. Second year, second issue, the sixth consecutive. 35 – 43.
19.Katakura A et al. 2007. Comparison of salivary cytokine levels in oral cancer patients and health subjects. Bull Tokyo Dent Coll. 48 (4): 199- 203.
20.Kohut ML, Senchina DS. 2004. Reversing age-associated immunosenescence via exercise. Exerc Immunol Rev, 10:6–41.
21.Mahon AD, Duncan GE, Howe CA and Del Corral P. 1997. Blood lactate and perceived exertion relative to ventilatory threshold: boys versus men. Med Sci Sports Exerc 29: 1332-1337.
22.Martinez LR and Haymes EM. 1992. Substrate utilization during treadmill running in prepubertal girls and women. Med Sci Sports Exerc 24: 975-983.
23.Miles D.S, Cox M.H, Bomze J.P, 1989. Cardiovascular responses to upper body exercise in normal and cardiac patients. Med Sci Sports Exerc. 21(5):126-131.
24.Moldoveanu AI, Shephard RJ, Shek PN. 2001. The cytokine response to physical activity and training. Sports Med, 31:115–44.
25.Nehlsen-Cannarella SL, Fagoaga OR, Nieman DC, Henson DA, Butterworth DE, Schmitt RL, et al. 1997. Carbohydrate and the cytokine response to 2.5 h of running. J Appl Physiol, 82:1662–7.
26.Nichol KL. 2005. Influenza vaccination in the elderly: impact on hospitalization and mortality. Drugs Aging, 22:495–515.
27.Pedersen B. K. 2000. Exercise and cytokines. Immunology and Cell Biology 78, 532–535.
28.Pedersen BK, Febbraio MA. 2008. Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6. Physiol Rev, 88:1379-406.
29.Pedersen BK, Fischer CP. 2007. Physiological roles of muscle-derived interleukin-6 in response to exercise. Curr Opin Clin Nutr Metab Care, 10:265-71.
30.Pedersen BK, Hoffman-Goetz L. 2000. Exercise and the immune system: Regulation integration and adaption. Physiol Reviews, 80:1055-81.
31.Pendergast D.R, 1989. Cardiovascular, respiratory and metabolic responses to upper body exercise. Med Sci Sports Exerc. 21(5): 121-5.
32.Pfaffe T, Cooper-White J, Beyerlein P, Kostner K, Punyadeera C. 2011. Diagnostic potential of saliva: current state and future applications. Clin Chem. 57(5): 675-87.
33.Santos R.V.T et al. 2007. Exercise, sleep and cytokines: Is there a relation? Sleep Medicine Reviews. 11, 231–239.
34.Sawka MN, Foley ME, Pimental NA, et al. 1983. Determination of maximal aerobic power during able-body exercise. J Appl Physiol. 54: 113-17.
35.Senchina D. S and Kohut M. L. 2007. Immunological outcomes of exercise in older adults. Clinical Interventions in Aging, 2(1) 3–16.
36.Søndergaard SR, Ostrowski K, Ullum H, Pedersen BK. 2000. Changes in plasma IL-6 and IL-1ra in response to adrenaline. Eur. J. Appl. Physiol. 83 (1): 95-8.
37.Starkie R, Ostrowski SR, Jauffred S, Febbraio M and Pedersen BK. 2003. Exercise and IL- 6 infusion inhibit endotoxin-induced TNF-alpha production in humans. FASEB J 17: 884-886.
38.Steensberg A, van Hall G, Osada T, Sacchetti M, Saltin B, Pedersen BK. 2000. Production of interleukin-6 in contracting human skeletal muscles can account for the exercise-induced increase in plasma interleukin-6. J Physiol 529: 237–242,.
39.Terra R et al. 2012. Effect of exercise on the immune system: response, adaptation and cell signaling. Rev Bras Med Esporte – Vol. 18, No 3. 208 – 214.
40.Timmons BW, Bar-Or O and Riddell MC. 2003. Oxidation rate of exogenous carbohydrate during exercise is higher in boys than in men. J Appl Physiol 94: 278-284.
41.Timmons BW. 2005. Paediatric exercise immunology: health and clinical applications. Exerc Immunol Rev 11: 108-144.
42.Timmons BW. 2006. Immune responses to exercise in children: A brief review. Ped Exerc Sci. Volume 18, Issue 3, August, 18, 290 – 299.
43.Toner M.M, Sawka M.N, Levine L, Pandolf K.B, 1983. Cardiorespiratory responses to exercise distributed between the upper and lower body. J Appl Physiol. 54 (5): 1403- 1407.
44.Usui T, Yoshikawa T, Ueda SH, et al. 2011. Effects of Acute Prolonged Strenuous Exercise on the Salivary Stress Markers and Inflammatory Cytokines. Jpn. J. Phys. Fitness Sports Med. 60(3): 295-304.
45.Vijayaraghava Ambarish and Radhika K. 2014. Alteration of Interferon Gamma (IFN-γ) in Human Plasma with Graded Physical Activity. Journal of Clinical and Diagnostic Research. Jun, Vol-8(6): 5- 7.
46.Williamson S et al 2012. Comparison of Biomarkers in Blood and Saliva in Healthy Adults. Nursing Research and Practice. 1- 4.
47.Sayedda K, Ahmed Q. Salivary Total Antioxidant Activity as a Non Invasive Biomarker for Oxidative Stress In Asthmatic Patients. National Journal of Integrated Research in Medicine. 2012;3(1):8-12.
48.uentsch A, Preshaw PM, Bremer-Streck S, Klinger G, Glockmann E, Sigusch BW. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment. Clinical oral investigations. 2008;12(4):345-52.