تاثیر فعالیت ورزشی هوازی بر بیان ژن های FNDC5و PGC1-α در رت های نر دیابتی

نویسندگان

1 دانشجوی دکتری بیوشیمی و متابولیسم ورزشی، دانشکده تربیت بدنی و علوم ورزشی ، دانشگاه گیلان، رشت ، ایران

2 استاد فیزیولوژی ورزشی ، دانشکده تربیت بدنی و علوم ورزشی ، دانشگاه تهران، تهران ، ایران

3 دانشیار فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی ، دانشگاه تهران، تهران ، ایران

4 دانشجوی دکتری فیزیولوژی ، دانشکده تربیت بدنی و علوم ورزشی ، دانشگاه تهران، تهران ، ایران

چکیده

تغییر فنوتیپ بافت چربی بر اثر تمرین تئوری جدیدی است که احتمالاٌ در نتیجه­ی بیان ژن FNDC5 عضلانی ایجاد می­شود. هدف از پژوهش حاضر بررسی تاثیر فعالیت ورزشی هوازی بر بیان ژن­های FNDC5 و PGC-1α رت­های نر دیابتی است. در این مطالعه 18 سر رت نر دیابتی (12 هفته سن و با وزن 220-240 گرم) به 3 گروه تقسیم شدند: بلافاصله پس از فعالیت ورزشی هوازی (AE0) (6سر)، 2ساعت پس از فعالیت ورزشی هوازی (AE2) (6سر) و کنترل (C) (5سر). هر دو گروه فعالیت ورزشی هوازی (AE) با سرعت vo2max65-60% به مدت 40 دقیقه­ به فعالیت روی نوارگردان پرداختند. برای بررسی بیان نسبی mRNA ژن­های FNDC5 و PGC-1α بافت عضلانی از روش Real time PCR استفاده شد. از آزمون ANOVA و تست تعقیبی توکی برای تحلیل داده­ها استفاده و سطح معنی­داری 05/0 در نظر گرفته­ شد. تحلیل داده­ها نشان داد که بین گروه­های تحقیق در بیان ژن­PGC-1α تفاوت معنی­داری وجود دارد (01/0P≤). نتایج آزمون توکی نشان داد،بیان ژن­PGC-1α در گروه بلافاصله پس از فعالیت ورزشی هوازی (AE0) نسبت به گروه کنترل افزایش معنی­داری داشت (01/0P). با این حال، بین گروه­های تحقیق در بیان ژن­FNDC5 تفاوت معنی­داری وجود نداشت (01/0P>). بنابراین، نتایج این تحقیق نشان داد که فعالیت ورزشی هوازی علارغم بیان PGC1-α تاثیری بر بیان ژن FNDC5 در رت­های نر دیابتی ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Aerobic Exercise on PGC-1α and FNDC5 Gene Expression in Diabetic Male Rats

نویسندگان [English]

  • Mousa khalafi 1
  • Ali Asghar Ravasi 2
  • Rahman Soori 3
  • Mohammad Moradi 4
1 PhD Student of Biochemistry and Sport Metabolism, Faculty of Physical Education and Sport Sciences, Guilan University, Rasht, Iran
2 Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Associate Professor of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
4 PhD Student of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
چکیده [English]

Change in adipose tissue phenotype caused by exercise training is a new theory manifested as the result of muscle FNDC5 gene expression. The aim of the present study was to investigate the effect of aerobic exercise on PGC-1α and FNDC5 gene expression of diabetic male rats. In this study, 18 diabetic male rats (12 weeks old, weight 220-240 gr) were divided into three groups: immediately after aerobic exercise (AE0) (n=6), 2 hours after aerobic exercise (AE2) (n=6) and control (C) (n=5). Both aerobic exercise (AE) groups exercised on the treadmill with 60-65% VO2max for 40 minutes. Real time PCR method was used to examine the mRNA relative expression of FNDC5 and PGC-1α genes of muscle tissue. ANOVA and Tukey post hoc test were used to analyze data at significance level of 0.05. Data analysis showed a significant difference in PGC-1α gene expression among the groups (P≤0.01.) Tukey test showed that PGC-1α gene expression significantly increased in the immediately after aerobic exercise (AE0) group compared to the control group (P≤0.01). However, there were no significant differences in FNDC5 gene expression among the groups (P>0.01). Therefore, the results of this study showed that despite PGC-1α expression, aerobic exercise has no effects on the FNDC5 gene expression in diabetic male rats.

کلیدواژه‌ها [English]

  • aerobic exercise (AE)
  • Gene expression
  • FNDC5
  • PGC-1α
  • diabetic male rats

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