تأثیر تمرین هوازی بر بیان ژن عامل هسته‌ای کبدی( HNF-4α )و مقاومت به انسولین در رت‌های دیابتی نوع 2

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، واحد تهران جنوب،دانشگاه آزاد اسلامی، تهران، ایران.

2 دانشیار، گروه فیزیولوژی ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران.

3 استاد، گروه فیزیولوژی ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران.

4 استادیار، گروه فیزیولوژی ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

 
هدف از پژوهش حاضر، بررسی اثر تمرین هوازی بر بیان ژن عامل هسته­ای کبدی (­ HNF-4α)، گلوکز، انسولین و مقاومت به انسولین در رت‌های دیابتی نوع دو بود. بدین‌منظور تعداد 20 سر رت نر 10 هفته­ای با وزن 20±220 گرم (در ابتدا) انتخاب و با تزریق درون‌صفاقی نیکوتین آمید-استرپتوزوسین دیابتی نوع دو شدند و به شیوۀ تصادفی به دو گروه تمرین هوازی و کنترل تقسیم شدند. برنامۀ تمرین هوازی به مدت 12 هفته و هر هفته 5 جلسه با زمان 10 تا 50 دقیقه و سرعت 18 تا 26 متر در دقیقه بود. گلوکز ناشتا، انسولین سرم و بیان ژن HNF-4α در بافت کبد هر دو گروه پس از آخرین جلسۀ تمرین اندازه­گیری و توسط آزمون تی مستقل با یکدیگر مقایسه شدند. نتایج نشان داد که تمرینات هوازی به کاهش معنا­دار گلوکز ناشتا (001/0=P)، مقاومت به انسولین (044/0=P) و افزایش معنا­دار انسولین (019/0=P) منجر شد. همچنین، بیان HNF-4α پس از تمرین هوازی در مقایسه با گروه کنترل کاهش معنا­داری داشت (007/0=P). براساس یافته­های موجود، احتمالاً تمرین هوازی می­تواند با افزایش انسولین سرم بیان ژن HNF-4α کبد رت­های دیابتی نوع دو را کاهش دهد. ازاین‌رو با کاهش بیان این ژن در اثر تمرین هوازی و به‌تبع آن کاهش گلوکز تولیدی کبد، ممکن است که یکی از مکانیسم­های اثر فعالیت ورزشی در افراد دیابت نوع دو باشد.

کلیدواژه‌ها


عنوان مقاله [English]

he Effect of Aerobic Exercise on Gene Expression of Hepatocyte Nuclear Factor-4α (HNF-4α) and Insulin Resistance in Type 2 Diabetic Rats

نویسندگان [English]

  • Elham Yadegari 1
  • Abdolali Banaeifar 2
  • Mohammadali Azarbaijani 3
  • Sajad Arshadi 4
1 PhD Student of Exercise Physiology, Department of Physical Education and Sport Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
2 Associate Professor, Department of Physical Education and Sport Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
3 Professor, Department of Physical Education and Sport Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
4 Assistant Professor, Department of Physical Education and Sport Sciences, South Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

 
The aim of this study was to investigate the effect of aerobic exercise on the gene expression of hepatocyte nuclear factor-4α (HNF-4α), glucose, insulin and insulin resistance in type 2 diabetic rats. For this purpose, 20 male 10-week-old male rats (weight 20±220 gr) were selected. Type 2 diabetes was induced intraperitoneally by the injection of nicotinamide-streptozotocin and then were randomly divided into two groups of aerobic exercise and control. The aerobic exercise program was performed for 12 weeks, 5 sessions a week, 10-50 minutes per session and speed of 18-26 meter/minute. Fasting glucose, serum insulin and HNF-4α gene expression in the liver tissue were measured in both groups after the last exercise session and compared by independent t test. The results showed that aerobic exercise significantly decreased fasting glucose (P=0.001), insulin resistance (P=0.044) and significantly increased insulin (P=0.019). Also, the expression of HNF-4α after aerobic exercise significantly decreased in comparison with the control group (P=0.007). Based on these findings, aerobic exercise is likely to reduce the liver HNF-4α gene expression of type 2 diabetic rats by increasing serum insulin. Thus, reduced expression of this gene through the effect of aerobic exercise and thereby reduced production of liver glucose may be one of the mechanisms of the effect of exercise in people with type 2 diabetes.

کلیدواژه‌ها [English]

  • Aerobic exercise
  • HNF-4α Gene Expression
  • Type 2 diabetes

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