تأثیر شش هفته تمرین تناوبی شدید بر بیان ژن و محتوای پروتئین‌های KIF5B و Dynein در هیپوکمپ موش‌های صحرایی نر ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی دانشکدۀ ادبیات و علوم انسانی دانشگاه لرستان، خرم‌آباد، ایران

2 استادیار گروه تربیت بدنی و علوم ورزشی دانشکدۀ ادبیات و علوم انسانی دانشگاه لرستان، خرم‌آباد، ایران

3 دانشیار گروه تربیت بدنی و علوم ورزشی دانشکدۀ ادبیات و علوم انسانی دانشگاه لرستان، خرم‌‌آباد، ایران

4 دانشیار گروه تربیت بدنی و علوم ورزشی دانشکدۀ ادبیات و علوم انسانی دانشگاه ولی‌عصر ( عج) رفسنجان، رفسنجان، ایران

چکیده

 
انتقال سیتوپلاسمی فرایندی حیاتی در دستگاه عصبی است که موجب بهبود بقای عصبی می‌شود. پروتئین‌های KIF5B و Dynein موتورهای حرکتی درگیر در انتقال سیتوپلاسمی هستند که نقش مهمی در انتقال رو به ­جلو و عقب محموله‌های مختلف دارند، اما تأثیر تمرینات ورزشی به‌ویژه تمرین تناوبی شدید (HIIT) بر بیان این پروتئین­ها کمتر بررسی شده است. ازاین‌رو در این پژوهش، تأثیر HIIT بر بیان ژن و میزان پروتئین‌های KIF5B و Dynein در هیپوکمپ موش‌های صحرایی نر ویستار بررسی شد. آزمودنی‌های پژوهش شامل 14 سر موش بود که به دو گروه تمرین (7) و کنترل (7) تقسیم شدند. برنامۀ تمرینی به مدت شش هفته و هر هفته پنج جلسه بر روی نوار گردان انجام گرفت. برای بررسی میزان پروتئین KIF5B و Dynein از روش ایمونوهیستوشیمی و برای اندازه‌گیری بیان mRNA متغیرهای پژوهش از روش Real-Time PCR استفاده شد. یافته‌های پژوهش نشان داد که شش هفته HIIT به کاهش معنا‌داری در بیان ژن KIF5B و Dynein (به‌ترتیب 001/0 P= و 001/0 P= و درصد تغییرات=072/-) منجر شد. همچنین در پی انجام شش هفته تمرین، کاهش معنا‌داری در محتوای KIF5B (001/0P=) و Dynein (004/0P=) مشاهده شد. این یافته‌ها بیانگر این مطلب است که HIIT با تنظیم کاهشی بیان ژن و محتوای پروتئینی KIF5B و Dynein در بافت هیپوکمپ مرتبط است، هرچند سازوکارهای پایه ناشناخته باقی مانده‌اند. این تغییرات نشان می‌دهد که HIIT ممکن است بر انتقال سیتوپلاسمی روبه‌جلو و روبه­عقب تأثیر منفی داشته باشد، چراکه انتقال سیتوپلاسمی به‌وسیلۀ KIF5B و Dynein میانجی‌گری می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of 6 Weeks of High Intensity Interval Training on the Gene Expression and Content of KIF5B and Dynein Proteins in Hippocampus of Male Wistar Rats

نویسندگان [English]

  • Hadi Kerendi 1
  • Rahim Mirnasoori 2
  • Masoud Rahmati 3
  • Abdolreza Kazemi 4
1 Ph.D. Student in Exercise Physiology, Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, Lorestan University, Khorramabad, Iran
2 Assistant Professor, Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, Lorestan University, Khorramabad, Iran
3 Associate Professor, Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, Lorestan University, Khorramabad, Iran
4 Associate Professor, Department of Physical Education and Sport Sciences, Faculty of Literature and Humanities, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran
چکیده [English]

Cytoplasmic transport is a vital process in the CNS that improves neuronal survival. KIF5B and Dynein are motor proteins involved in cytoplasmic transport which play an important role in the anterograde/retrograde transport. However, the effect of training especially high intensity interval training (HIIT) is less clear on the expression of these proteins. The present study examined the effect of HIIT on the gene expression and the amount of KIF5B and Dynein proteins in the hippocampal tissue of Wistar male rats. 14 rats were divided into 2 groups: (1) the training (TG: n=7) and (2) the control (CG: n=7). Training program was carried out on a treadmill for 6 weeks, 5 sessions per week. The protein contents of KIF5B and Dynein were determined by the immunohistochemical analysis. Moreover, the Real-Time polymerase chain reaction (Real-Time PCR) procedure was used to measure mRNA expression of the variables. The findings showed that 6 weeks of HIIT significantly decreased the gene expression of KIF5B and Dynein (P=0.001 and P=0.001 or percentage of changes= -0.72 respectively). Also, HIIT significantly decreased KIF5B (P=0.001) and Dynein (P=0.004) protein content after 6 weeks of HIIT. These findings demonstrated that HIIT was associated with the down-regulation of gene expression and protein content of KIF5B and Dynein in the hippocampal tissue although the underlying mechanisms have remained unknown. These changes show that HIIT may have negative effects on anterograde/retrograde cytoplasmic transport because the cytoplasmic transport is mediated by KIF5B and Dynein.

کلیدواژه‌ها [English]

  • Dynein
  • HIIT
  • KIF5B
  • Cytoplasmic Transport
  • Hippocampus

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پژوهش های فیزیولوژی و مدیریت در ورزش
دوره 10، شماره 3
آذر 1397
صفحه 123-137

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