The Effect of 8 Weeks of Aerobic Exercise on the Expression of PGC-1α and VEGF Genes in Healthy Male Rats' Myocardial Muscle

Authors

1 Ph.d Student of Shiraz University, Fars.Iran

2 Assistant Professor, Department of Exercise Physiology, University of Tehran, Tehran, Iran

3 MSc of Exercise Physiology, Endocrine Physiology Research Center (EPRC), Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Associate Professor, Cellular-Molecular Research Center (CMERC), Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

The most important mitochondrial biogenesis regulator is PGC-1α that is a cell receptor and facilitates the mitochondrial protein release, while VEGF is the most important growth factor involved in this process. The aim of this study was to investigate the effect of 8 weeks of aerobic training on the expression of PGC-1α and VEGF genes in male healthy rats' myocardial muscle by fundamental experimental method. 12 male Wistar rats (mean weight 180±20 gr and age 8 weeks) were divided into control (n=6) and exercise (n=6) groups. The exercise group had 30 minutes of running on a treadmill with an intensity of 50-60 VO2max for 8 weeks, 5 sessions per week while the control group had ran on the treadmill for 15 minutes with 2 m/min. speed. On the day after the last session, rats were dissected and the samples were transferred to the laboratory to extract RNA. To investigate the expression of PGC1 and VEGF genes, SYBER Green Real-time PCR technique was used. REST software (version 2009) was used to determine the relative expression of exercise group relative to the control group (P˂0.05). The expressions of PGC-1α and VEGF genes were 0.97 and 0.85 in exercise group relative to the control group (not statistically significant, P=0.95 and P=0.63 respectively). The results indicated the insignificant change in the expression of PGC-1α and VEGF genes in myocardial muscle following 8 weeks of aerobic exercise.

Keywords

References

1.Aoi, W., Naito, Y., Mizushima, K., Takanami, Y., Kawai, Y., Ichikawa, H., & Yoshikawa, T. (2010). The microRNA miR-696 regulates PGC-1α in mouse skeletal muscle in response to physical activity. American Journal of Physiology-Endocrinology and Metabolism. 298(4): E799-E806.
2.Chinsomboon J,Ruas J,Gupta R, et al.2009.The transcriptional coactivator PGC-1α mediates exercise-induced angiogenesis in skeletal muscle. Proceeding of the National Academy of Sciences. 106(50), pp: 21401-21406.
3.Christopoulos, A, Ahn, A, Klein, J.D,et al.(2011). Biology of vascular endothelial growth factor and its receptors in head and neck cancer: Beyond angiogenesis. Head and neck.33: pp: 1220-1229.
4.Coffey, V. G., & Hawley, J. A. (2007).The molecular bases of training adaptation. Sports medicine. 37(9): pp: 737-763.
5.Ding, Y. H., Luan, X. D., Li, J., Rafols, J. A., Guthinkonda, M., Diaz, F. G., & Ding, Y. (2004). Exercise-induced overexpression of angiogenic factors and reduction of ischemia/reperfusion injury in stroke. Current neurovascular research, 1(5), 411-420.
6.Eggintons,H, Hudlicka o, Brown,M.D, et al. (1998). Capillary growth in relation to blood flow and performance in overload rat skeletal muscle. Apply Physiology.85: pp: 2025-2032.
7.Haram, P.M, Kemi,J, Lee,S.J, et al.(2009). Aerobic interval training vs. continuous moderate exercise in the metabolic syndrome of rats artificially selected for low aerobic capacity. Cardiovasc Res.81: pp: 723-732.
8.Hoydal M A, Wisloff U,Kemi O J, et al. (2007). Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Cardiovascular Prevention & Rehabilitation.14(6): pp: 753-760.
9.Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T. (2006). Exercise training improves aging-induced downregulation of angiogenic signaling cascade in hearts. Am J Physiol Heart Circ Physiol . 291: pp:1290-1298.
10.Jensen,L , Pilegaard,H , Neufer,P.D. (2004). Effect of acute exercise and exercise training on VEGF splice variants in human skeletal muscle. Molecular Human Reproduction. 282: pp: 335-398.
11.Little, J. P., Safdar, A., Wilkin, G. P., Tarnopolsky, M. A., & Gibala, M. J .(2010). A practical model of low-volume high-intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanism. J Physiol. 588(6): pp: 1011-1022
12.Nourshahi,M, Hedayati,M, Nemati,J,et al.(2012). Effect of 8 weeks endurance training on serum vascular endothelial growth factor and endostatin in Wistar rats. Koomesh.13(4):pp: 474-479.
13.Pilegaard,H, Saltin,B, Neufer,D. (2003). Exercise induces transient transcriptional activation of thePGC-1agene in human skeletal muscle . J Physiol. 546(3): pp: 851–858.
14.Ranjbar K, Nourshahi M, Hedayati M, Taheri H. (2011). Effect of gender and physical activity on serum vascular endothelial growth factor at rest and response of submaximal exercise. Iran J Endocrinol Metab . 3: pp: 294-300.
15.Rullman E, Rundqvist H, Wågsäter D, Fischer H, Eriksson P, Sundberg CJ, et al. A (2007).single bout of exercise activates matrix metalloproteinase in human skeletal muscle. J Appl Physiol . 102: pp: 2346-2351.
16.Russell, A. P., Feilchenfeldt, J., Schreiber, S., Praz, M., Crettenand, A., Gobelet, C, et al. (2003). Endurance Training in Humans Leads to Fiber Type-Specific Increases in Levels of Peroxisome Proliferator-Activated Receptor-γ Coactivator-1 and Peroxisome Proliferator-Activated Receptor-α in Skeletal Muscle. Diabetes. 52 (12): pp: 2874 –2881.
17.Terada, S., Goto, M., Kato, M., Kawanaka, K., Shimokawa, T., & Tabata, I. (2002). Effects of low-intensity prolonged exercise on PGC-1 mRNA expression in rat epitrochlearis muscle. Biochemical and biophysical research communications, 296(2): pp: 350-354.
18.Tesch, A. (2014). Aerobic exercise does not compromise muscle. J Appl Physiol, 116(6): pp: 611-620.
19.Tunstall RJ, Mehan KA, Wadley GD, Collier GR, Bonen A,Hargreaves M & Cameron-Smith D (2002). Exercise trainingincreases lipid metabolism gene expression in human skeletalmuscle. Am J Physiol Endocrinol Metab. 283: pp: 66–72.
20.Wu Z, Puigserver P, Andersson U, Zhang C, Adelmant G, Mootha Vet al. (1999). Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell. 9;98(1): pp:115-24.

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