Document Type : Original Article
Authors
1
PHD student of Exercise Physiology, Department of Physical Education, Tabriz
2
Assistant Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
3
Associate Prof., Department of Exercise Physiology, Tabriz branch, Islamic Azad University, Tabriz, Iran
4
Assistant Prof., Department of Vet, Tabriz branch, Islamic Azad University, Tabriz, Iran
5
Associate Professor of Exercise Physiology, Azarbaijan Shahid Madani University, Tabriz, Iran
Abstract
Aim: Arsenic exposure leads to cardiomyocyte apoptosis as well as cardiac failure and it seems high intensity interval training (HIIT) and curcumin supplementation could be beneficial. However, no straight information is available in this area. Therefore, the effects of HIIT and curcumin supplementation on myocardial caspase 3 level as well as expression of miR-30, miR-199a and miR-874 were investigated in rats exposed to arsenic via drinking water.
Methods: 48 rats were randomized in six groups including Arsenic-training, Arsenic-curcumin, Arsenic-training+curcumin, arsenic, ethanol control and normal control. Arsenic 5 mg/bw.day and curcumin 15 mg/bw.day, were consumed orally for entire the study period. HIIT were conducted for six weeks (5 d/w, 60 min/session (with 4 min running at 85-90% of Vo2max and 2 min recovery at 50-60% of Vo2max intervals).
Results: Arsenic exposure increased cardiac caspase 3 level (P=0.001), while decreased mir-30 expression (P=0.001) compared to control group and these changes could only attenuated by curcumin supplementation (P=0.039 and P=0.003 respectively). However, HIIT did not exacerbate effects from arsenic exposure upon caspase3 up regulation as well as a diminished mir-30 expression level (P=0.095 and P=0.26 respectively).
Conclusion: Arsenic exposure could likely predisposes the heart toward apoptosis which seems could be attenuated only by curcumin supplementation. Moreover it seems that HIIT fails to overcome arsenic toxicity; neither exuberate cardiac apoptosis. However, more researches are warranted because of the lack of evidence and also study limitations.
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