Sport Physiology & Management Investigations

Sport Physiology & Management Investigations

The effect of an aerobic exercise course on the genes expression of Sirt-1 and PGC-1α in the left ventricle of rats with type 2 diabetes

Document Type : Original Article

Authors
Seyedeh somayeh samaie 1
Ali Asghar Ravasi 2
Siroos Choobineh 3
Maryam Delfan 4
1 Phd Student in Sport Physiology, Alborz Campus, University of Tehran, Tehran, Iran.
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran. E-mail address: aaravasi@ut.ac.ir
3 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran
4 Assistant Professor, Department of Exercise Phyiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
Abstract
introduction:Regular aerobic exercise with proportionate intensity volume improved gene expression in the left ventricle of diabetic pations.The purpose of this study was to investigation of an aerobic exercise course on the genes expression of Sirt-1 and PGC-1α in the left ventricle of rats with type 2 diabetes.
methods:in the present experimental study, 18 diabetic male mice were divided into three groups of 6;Normal Control(NC), Diabetic Control(DC), Aerobic exercise(ET). diabetes was induced in all groups expected non diabetic control group by interaperitoneal injection of sereptozotocin(STZ) after12 hours fasting. glucose concentration was measured by glucose oxidase method. ELISA method was used to measure the insulin and HOMA-IR method was used to measure insulin resistance index.the expression of Sirt-1 and PGC-1α genes was determined by Real time- PCR and the groups were compared by one way anova test at the alpha level of 0.05.
Results:the genes expression of Sirt-1 and PGC-1α genes in the exercise group increased significantly compared to diabetic control(P= 0.001) and(P= 0.02), respectively.glucose index and insulin resistance were decreased in the exercise group.
Conclusion: aerobic exercise by regulating the genes expression of Sirt-1 and PGC-1α in the left ventricle of diabetic rats, could possibly improved mitochondrial biogenesis.
Keywords
aerobic exercise training
Sirt-1
PGC-1α
insulin resistance
  1. Henle T. AGEs in foods: do they play a role in uremia? Kidney international. 2003;63:S145-S7.
  2. Li W, Zho P, Wang G, Lu X, Jiang Y, Zhao X. Anti-inflammatory effects of lycopene prevents cardiac dysfunction in streptozotocin-diabetic rats. Int J Clin Exp Med. 2016;9(5):8047-54.
  3. Condorelli G, Roncarati R, Ross Jr J, Pisani A, Stassi G, Todaro M, et al. Heart-targeted overexpression of caspase3 in mice increases infarct size and depresses cardiac function. Proceedings of the National Academy of Sciences. 2001;98(17):9977-82.
  4. Long AN, Dagogo‐Jack S. Comorbidities of diabetes and hypertension: mechanisms and approach to target organ protection. The journal of clinical hypertension. 2011;13(4):244-51.
  5. Condorelli G, Roncarati R, Ross J, Pisani A, Stassi G, Todaro M, et al. Heart-targeted overexpression of caspase3 in mice increases infarct size and depresses cardiac function. Proceedings of the National Academy of Sciences. 2001;98(17):9977-82.
  6. Seale P, Kajimura S, Yang W, Chin S, Rohas LM, Uldry M, et al. Transcriptional control of brown fat determination by PRDM16. Cell metabolism. 2007;6(1):38-54.
  7. Scheffer DL, Silva LA, Tromm CB, da Rosa GL, Silveira PC, de Souza CT, et al. Impact of different resistance training protocols on muscular oxidative stress parameters. Applied Physiology, Nutrition, and Metabolism. 2012;37(6):1239-46.
  8. Cai L, Wang Y, Zhou G, Chen T, Song Y, Li X, et al. Attenuation by metallothionein of early cardiac cell death via suppression of mitochondrial oxidative stress results in a prevention of diabetic cardiomyopathy. Journal of the American College of Cardiology. 2006;48(8):1688-97.
  9. González-Montero J, Brito R, Gajardo AI, Rodrigo R. Myocardial reperfusion injury and oxidative stress: Therapeutic opportunities. World Journal of Cardiology. 2018;10(9):74.
  10. Guarente L, editor Sirtuins in aging and disease. Cold Spring Harbor symposia on quantitative biology; 2007: Cold Spring Harbor Laboratory Press.
  11. Kemp K, Griffiths J, Campbell S, Lovell K. An exploration of the follow-up up needs of patients with inflammatory bowel disease. Journal of Crohn's and Colitis. 2013;7(9):e386-e95.
  12. Mortensen OH, Frandsen L, Schjerling P, Nishimura E, Grunnet N. PGC-1α and PGC-1β have both similar and distinct effects on myofiber switching toward an oxidative phenotype. American Journal of Physiology-Endocrinology and Metabolism. 2006;291(4):E807-E16.
  13. Bruce CR, Anderson MJ, Carey AL, Newman DG, Bonen A, Kriketos AD, et al. Muscle oxidative capacity is a better predictor of insulin sensitivity than lipid status. The Journal of Clinical Endocrinology & Metabolism. 2003;88(11):5444-51.
  14. of Inflammation M. Retracted: Treadmill Training Increases SIRT-1 and PGC-1α Protein Levels and AMPK Phosphorylation in Quadriceps of Middle-Aged Rats in an Intensity-Dependent Manner. Mediators of Inflammation. 2017;2017.
  15. Liang H, Ward WF. PGC-1α: a key regulator of energy metabolism. Advances in physiology education. 2006.
  16. Huang C-Y, Yang A-L, Lin Y-M, Wu F-N, Lin JA, Chan Y-S, et al. Anti-apoptotic and pro-survival effects of exercise training on hypertensive hearts. Journal of applied physiology. 2012;112(5):883-91.
  17. Palmer BF, Clegg DJ. Physiology and pathophysiology of potassium homeostasis. Advances in physiology education. 2016.
  18. Gordon LA, Morrison EY, McGrowder DA, Young R, Fraser YTP, Zamora EM, et al. Effect of exercise therapy on lipid profile and oxidative stress indicators in patients with type 2 diabetes. BMC complementary and alternative medicine. 2008;8(1):1-10.
  19. Wang Y, Wu Y, Wang Y, Xu H, Mei X, Yu D, et al. Antioxidant properties of probiotic bacteria. Nutrients. 2017;9(5):521.
  20. Golbidi S, Badran M, Laher I. Antioxidant and anti-inflammatory effects of exercise in diabetic patients. Experimental diabetes research. 2011;2012.
  21. Marton O, Koltai E, Takeda M, Koch LG, Britton SL, Davies KJ, et al. Mitochondrial biogenesis-associated factors underlie the magnitude of response to aerobic endurance training in rats. Pflügers Archiv-European Journal of Physiology. 2015;467(4):779-88.
  22. Khakdan S, Delfan M, Heydarpour Meymeh M, Kazerouni F, Ghaedi H, Shanaki M, et al. High-intensity interval training (HIIT) effectively enhances heart function via miR-195 dependent cardiomyopathy reduction in high-fat high-fructose diet-induced diabetic rats. Archives of physiology and biochemistry. 2020;126(3):250-7.
  23. PITHON-CURI TNC. Aprogram Of Moderate Physical Training For Wistar Rats Based On Maximal Oxygen Consumption. Journal of strength and conditioning research. 2007;21(3):000-.
  24. Høydal MA, Wisløff U, Kemi OJ, Ellingsen Ø. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. European Journal of Preventive Cardiology. 2007;14(6):753-60.
  25. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome biology. 2002;3(7):1-12.
  26. Kotenko SV, Gallagher G, Baurin VV, Lewis-Antes A, Shen M, Shah NK, et al. IFN-λs mediate antiviral protection through a distinct class II cytokine receptor complex. Nature immunology. 2003;4(1):69-77.
  27. Bartlett JD, Hwa Joo C, Jeong T-S, Louhelainen J, Cochran AJ, Gibala MJ, et al. Matched work high-intensity interval and continuous running induce similar increases in PGC-1α mRNA, AMPK, p38, and p53 phosphorylation in human skeletal muscle. Journal of applied physiology. 2012;112(7):1135-43.
  28. Saleem A, Adhihetty PJ, Hood DA. Role of p53 in mitochondrial biogenesis and apoptosis in skeletal muscle. Physiological genomics. 2009;37(1):58-66.
  29. Bækkerud FH, Salerno S, Ceriotti P, Morland C, Storm-Mathisen J, Bergersen LH, et al. High intensity interval training ameliorates mitochondrial dysfunction in the left ventricle of mice with type 2 diabetes. Cardiovascular toxicology. 2019;19(5):422-31.
  30. Cunha VN, de Paula Lima M, Motta‐Santos D, Pesquero JL, de Andrade RV, de Almeida JA, et al. Role of exercise intensity on GLUT4 content, aerobic fitness and fasting plasma glucose in type 2 diabetic mice. Cell biochemistry and function. 2015;33(7):435-42.
  31. Oliveira NR, Marques SO, Luciano TF, Pauli JR, Moura LP, Caperuto E, et al. Treadmill training increases SIRT-1 and PGC-1α protein levels and AMPK phosphorylation in quadriceps of middle-aged rats in an intensity-dependent manner. Mediators of inflammation. 2014;2014.
  32. Gurd BJ, Perry CG, Heigenhauser GJ, Spriet LL, Bonen A. High-intensity interval training increases SIRT1 activity in human skeletal muscle. Applied Physiology, Nutrition, and Metabolism. 2010;35(3):350-7.
  33. Dela F, Handberg A, Mikines KJ, Vinten J, Galbo H. GLUT 4 and insulin receptor binding and kinase activity in trained human muscle. The Journal of physiology. 1993 Sep 1;469(1):615-24.
  34. Sarga L, Hart N, Koch L, Britton S, Hajas G, Boldogh I, et al. Aerobic endurance capacity affects spatial memory and SIRT1 is a potent modulator of 8-oxoguanine repair. Neuroscience. 2013;252:326-36.