Investigating the effects of melilot extract and resistance training on protein expression APP in the hippocampus of Alzheimer's model rats

Alzheimer's disease is one of the major health problems of society, which may be caused by inflammatory and genetic changes. Considering the problems of memory, performance, the suffering of the patient and his family, and paying exorbitant fees, it is felt necessary to investigate methods to reduce these problems. Therefore, researchers have proposed solutions such as physical activity and exercise and the use of supplements or herbal medicines. The purpose of this study was to investigate the effects of Melilotus officinalis and a period of resistance training on the expression of APP genes in the hippocampus of rats with Alzheimer's disease. 55 male Wistar rats at the age of eight weeks were obtained from the Pasteur Institute. Then the rats were randomly divided into 5 groups: healthy control group, Alzheimer's control group, Alzheimer's group + resistance training, Alzheimer's group + Melilotus officinalis, Alzheimer's group + Melilotus officinalis + resistance exercise. Alzheimer's was induced by beta-amyloid peptide 42-1. The resistance training protocol was considered as an intervention (training was done for eight weeks, three sessions a week, and in an increasing form, by carrying weights from a 26-step ladder), and the training groups were accurately weighed every week.
The load of each rat was selected based on its weight. Melilotus officinalis was used as a supplement at the dose of 300 mg per kilogram of body weight for 6 weeks and 5 days per week as an intraperitoneal injection. Morris's water maze test was used to test spatial memory. 72 hours after the last training session, rats were anesthetized. Hippocampus samples analyzed . According to the results of ANOVA, there is a significant increase in APP mRNA between the groups . The results of Bonferroni's post hoc test showed that there is a significant difference in the APP mRNA variable between the control group and the Alzheimer's group, Melilotus officinalis, and resistance training . There was a significant decrease in the time spent in the Barnes Maze to find the target well by rat between the Alzheimer's control group, the Alzheimer's group + Melilotus officinalis + resistance training and the Alzheimer's group + resistance training . The results of the present study showed that the supplement of Melilotus officinalis along with resistance training probably reduced oxidative stress and inflammatory factors, released neurotrophins stimulated neurogenesis in the structure of the hippocampus, and prevented the reduction of APP gene expression caused by Alzheimer's.