The Effect of 8 Weeks ofEndurance Training and High Intensity Interval Training on Gene Expression of MEF2A and MEF2Din Obese Male Wistar Rats

Document Type : Original Article

Authors

1 Associate Professor of Exercise Physiology, Payame Noor University, Tehran, Iran

2 Assistant Professor of Exercise Physiology, Physical Education Faculty, ShahidBeheshti University, Tehran, Iran

Abstract

The aim of this study was to investigate the effect of 8 weeks of endurance training (ET) and high intensity interval training(HIIT)on gene expression of MEF2A and MEF2Din obese male Wistarrats. For this purpose, 27 Wistar rats (35-45 days old,mean weight 110+10 g)were divided into 3 groups: obese control with no intervention (n=5), ET (n=8) and HIIT (n=8). At the beginning of the study, samples were collected from 6 rats and 21 rats had a high-fat diet for 8 weeks. When the body weight reached above 310 gbased on Lei index, animals were prepared for the endurance training and HIIT protocols. 48 hours after the last training session in which rats were fasted for 8 hours, gastrocnemius muscle biopsy was performed and they were frozen at -80°C. Gene expression of MEF2A and MEF2D were evaluatedusing PCR-Real Time, descriptive statistics including mean+SD and one-way ANOVA and independent t test after ensuring normal data distribution by KS test (P<0.05). The results showed that 8 weeks of ET and HIIT had no significant effects on MEF2A gene expression in gastrocnemius muscle of obese rats (P=0.068) but 8 weeks of HIIT increased significantlyMEF2D gene expression of this muscle in obese rats (P=0.042). It seems that HIIT protocol in obese male rats can have a beneficial effect on the glucose profile in diabetic conditionsso it can be used by sports specialist and physicians for diabetic patients as a part of their treatment process.

Keywords

References

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