The main problem in both developed and undeveloped countries in the public health sector is physical inactivity. On the other hand, FIFA research results provide compelling reasons for promoting soccer as an exercise that has improved health as well as social behavior. The present study intended to investigate the effect of 6 weeks of SSGs on some health indicators and telomere length in men aged between 35 and 41. In this semi-experimental study, the statistical population consisted of men aged between 35 and 41 in 2 groups: control (n=8) and experimental (n=10). The subjects played soccer according to the exercise protocol for six weeks, two 45-minute sessions per week. To check the intergroup data, independent sample t test was used and paired sample t test with SPSS 25 was applied for within-group data at significance level of P<0.05. Real Time PCR was used to measure the telomere length; the fat percentage was measured by the caliper and the VO_2max by the Rockport Field Test. The results indicated that there was a significant change in the fat percentage and VO_2max following 6 weeks of SSGs while no significant changes were observed in the BMI, telomere length and weight. It can be cautiously concluded that 6 weeks of SSGs improves health in men aged between 35 and 41 by affecting their fat percentage and VO_2max and does not have an effect on their telomere length.