The Effect of Two Intensities of Interval Training on Gene Expression of MyoD and Pax7 in Soleus Muscle of Myocardial Infarcted Wistar Rats

Document Type : Original Article

Authors

1 PhD of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Alborz Branch, University of Tehran, Karaj, Iran

2 Associate Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

Abstract

Myocardial infarction is a disease that not only influences cardiovascular system, but also influences other body systems including skeletal muscles. Thus, the aim of this study was to examine two type of interval trainingwith low and high intensity on gene expression of MyoD and Pax7. 24 Wistar rats (age: 10 weeks) were selected and underwent a coronary artery blocking surgery. Eventually, they were randomly divided into 4 groups: low intensity training (LIT) (n=6), high intensity training (HIT) (n=6), sham (n=6) and control (con) (n=6). 4 weeks after operation, subjects trained for 6 weeks, 3 session per week. After 6th week, subjects were anesthetized and killed and their soleus muscle were separated for further analysis. Data from RT-PCR technique were quantified by ΔΔCt formula and analyzed by one-way ANOVA test after Shapiro-Wilk and Leven tests. The results showed no significant differences in the effect of training intensities on MyoD and Pax7 among groups. However, it has shown that training has positive effects of cardiovascular system and muscular disorders resulted from MI, further studies with more control of other effective factors are required for a definite comment on the effects of different types of training on markers such as MyoD and Pax7 which are highly associated with treating muscle atrophy.

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