Sport Physiology & Management Investigations

Sport Physiology & Management Investigations

The Effect of Aquatic High-Intensity Interval Training on Antioxidants and Oxidative Stress in the Hippocampus of Parkinsonian Rats

Document Type : Original Article

Authors
Hasan Estakhr 1
Sarah Hojjati 2
Mehrzad Moghadasi 2
Mohammad Amin Edalatmanesh 3
1 Exercise physiology Department, Shiraz branch, Islamic Azad University, Shiraz, Iran.
2 Department of exercise physiology, Shiraz branch, Islamic Azad University, Shiraz, Iran
3 Associate professor in physiology, Department of biology, Shiraz branch, Islamic Azad University, Shiraz, Iran
10.22034/spmi.2024.461178.2612
Abstract
Background and Aim

Oxidative stress is responsible for the progression of Parkinson Disease (PD). This study aimed to investigate the effect of Aqua High-Intensity Interval Training (HIIT) on Malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT), and the SOD/CAT ratio.

Materials and Methods

Twenty-three male Wistar rats (9±1 weeks, 200±2.1 grams) were enrolled in this experimental study. Fifteen rats were randomly grouped into Parkinson (n=8) or Parkinson-Training (n=7) after the induction and confirmation of PD (via reserpine, 1 mg/kg, for 4 consecutive days, IP). Eight rats were designated as the Control group. The Catalepsy test and histopathological assays were used for PD confirmation. The Parkinson-training group underwent 6 weeks of HIIT (20 workouts of 30 seconds with overload and 30 seconds of rest between workouts). The hippocampus was then removed for biochemical analysis.

Results

The tissue level of MDA increased in the Parkinson group compared to the Control (p=0.002) and decreased in the Parkinson-training group compared to the Parkinson group (p=0.034). Tissue levels of SOD and CAT decreased in the Parkinson group compared to the Control (p≤0.001) and increased in the Parkinson-training group compared to the Parkinson group (p≤0.001). There was a significant reduction in the SOD/CAT ratio in the Parkinson-training group compared to the Parkinson group (p≤0.001).

Conclusion

HIIT may improve PD by increasing antioxidant biomarkers and decreasing oxidative stress.
Keywords
Parkinson Disease
Antioxidants
High-Intensity Interval Training
Catalase