Excessive accumulation of fat leads to obesity, which is also known as enlarged adipose tissue. This process is caused by fat hyperplasia or fat tissue hypertrophy. Obesity is associated with chronic inflammation and increased inflammatory indices. The aim of this study is to investigate the effect of 10 weeks of high intensity interval training (HIIT) and high fat diet (HFD) on IL-6 and TNF-alpha in adipose tissue of male Wistar rats. Research method: 24 male Wistar rats were divided into four groups: control (normal diet), intense interval training (HIIT), high-fat diet (HFD) and intense interval training + high-fat diet (HIT+HFD). Before the start, the maximum running speed test was taken. Then the rats were exposed to interventions for 10 weeks and after the end of this period, the visceral fat tissue was separated and stored at -20 temperature for laboratory analysis. Findings: 10-week high-fat diet (HFD) significantly increased IL-6 and TNF-alpha levels in adipose tissue. This increase was also observed in the group of intense interval training + high-fat diet (HIT+HFD) compared to the control group, but it was less than the high-fat diet (HFD) (p<0.05). Also, the levels of IL-6 and TNF-alpha in adipose tissue in response to HIIT compared to the control group had a non-significant decrease (p<0.05). Fat profiles (cholesterol, triglycerides, LDL, VLDL and HDL) were also significantly increased in two groups consuming HFD and HIT+HFD, and in response to HIIT, HDL levels increased significantly (<0.05). p). Conclusion: HFD causes an increase in the expression of inflammatory cytokines in the body, and exercising can only partially moderate this increase. Adherence to diet and exercise are recommended to fully control inflammatory indicators in the body.