بررسی میزان تغییرات آنزیم‌های آنتی‌اکسیدانی بافت ریه پس از تمرین استقامتی همراه با مصرف بربرین کلراید در رت‌های ویستار دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، دانشگاه آزاد اسلامی واحد بروجرد، بروجرد، ایران

2 دانشیار، دانشکدۀ علوم ورزشی، دانشگاه رازی، کرمانشاه، ایران

3 استاد، دانشکدۀ علوم ورزشی، دانشگاه آزاد اسلامی واحد تهران مرکز، تهران، ایران

4 دانشیار، دانشکدۀ پزشکی، دانشگاه علوم پزشکی شهید صدوقی، یزد، ایران

چکیده

 
تمرین هوازی و بربرین کلراید هر دو خاصیت آنتی‌اکسیدانی دارند، بنابراین پژوهش حاضر درصدد بررسی میزان تغییرات آنزیم‌های آنتی‌اکسیدانی بافت ریه پس از تمرین استقامتی همراه با مصرف بربرین کلراید در رت‌های ویستار دیابتی بود. به این منظور، 40 سر رت نر بالغ نژاد ویستار 200 تا250 گرمی به‌صورت تصادفی در 5 گروه کنترل سالم، کنترل دیابتی (با تزریق STZ)، تجربی دیابتی+تمرین بدنی، تجربی دیابتی+مصرف بربرین (mg/kg 50)، تجربی دیابتی+تمرین بدنی+ مصرف بربرین قرار گرفتند. 24 ساعت پس از آخرین جلسۀ تمرین، حیوانات با استفاده از تزریق درون‌صفاقی کتامین بی‌هوش شده و بافت ریه به‌منظور سنجش تغییرات آنزیم‌های آنتی‌اکسیدانی سوپراکسید دیسموتاز، کاتالاز و گلوتاتیون پراکسیداز جدا شد. از آزمون تی زوجی و آزمون تحلیل واریانس به‌منظور تجزیه‌وتحلیل داده‌ها با استفاده از نرم‌افزار SPSS (نسخۀ 22) و در سطح 05/0=α استفاده شد. نتایج نشان داد که نسبت به گروه کنترل میزان فعالیت‌های آنزیم CAT، SOD و GPX در تمامی گروه‌های تجربی به‌طور معناداری بالاتر بود (001/0=P). اثر مداخله در گروه دیابتی+تمرین+بربرین در مقایسه با گروه دیابتی+تمرین و گروه دیابتی+بربرین به‌طور معناداری بالاتر بود. نتیجۀ نهایی اینکه تمرین استقامتی و مصرف بربرین کلراید می‌تواند سبب افزایش معنادار فعالیت آنزیم‌های آنتی‌اکسیدانی بافت ریه در رت‌های دیابتی شود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Changes in Lung Tissue Antioxidant Enzymes after Endurance Training with The Berberine Chloride Usage in Diabetic Wistar Rats

نویسندگان [English]

  • Elham Farhadfar 1
  • Naser Behpour 2
  • Mohammad Ali Azarbaaeijani 3
  • Ali Moradi 4
1 Ph.D student, Islamic Azad University, Boroujerd Branch, Boroujerd, Iran
2 Associate Professor, Faculty of physical education and sport sciences. Razi University, Kermanshah, Iran
3 Professor, Islamic Azad University, Tehran Branch, Tehran, Iran
4 Associate Professor, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
چکیده [English]

 
Aerobic training and berberine chloride both have antioxidant characteristics, therefore the present study aimed to evaluate the changes in lung tissue antioxidant enzymes after endurance training with the use of berberine chloride in diabetic vestibular rats. For this purpose, 40 adult male Wistar rats weighing approximately 200-250 g were randomly divided into 5 groups including healthy control ,diabetic control group (with STZ injection), diabetic experimental group+physical Exercise, diabetic experimental group + berberine Intake (50 mg/kg), diabetic experimental group+physical exercise+berberine Intake. 24 hours after the last exercise session, the animals were anesthetized with an intraperitoneal injection of Ketamine and lung tissue was harvested to measure the antioxidant enzyme activity of superoxide dismutase, catalase, and glutathione peroxidase. Paired t-test and ANOVA were used to analyze the data using SPSS software (version 22) with significant level α=0.05. The results showed that compare to control group, the level of CAT, SOD and GPX activity was significantly higher in all of the experimental groups (P=0.001). In comparison to the diabetic + training group and the diabetic + berbine group, the effects of intervention was significantly higher in the diabetic+training+berberine group. The final conclusion indicated that endurance training with berberin chloride consumption can cause a significant increase in the anti-oxidant enzyme activity in the lung tissue of diabetic rats.

کلیدواژه‌ها [English]

  • Berberine chloride
  • Catalase
  • Diabetes mellitus
  • Glutathione peroxidase
  • Superoxide dismutase

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پژوهش های فیزیولوژی و مدیریت در ورزش
دوره 13، شماره 2
شهریور 1400
صفحه 153-162

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