Oxidative stress has been reported as an increase in reactive oxygen species in breast cancer, and physical activity can also prevent breast cancer recurrence by enhancing the antioxidant system. This study aimed to investigate the role of 12 weeks of high-intensity interval training (HIIT) and moderate–intensity continuous training (MICT) on serum levels of GPX and SOD in breast cancer survivors. in this quasi-experimental study, 45 postmenopausal women (age: 57.25±3.91) who after the termination of breast cancer treatment, were randomly divided into three groups of 15 individuals each (1) a high-HIIT group, and (2) MICT were performed for 12 weeks, three days a week, using a cycle ergometer. Biochemical indices were evaluated 48 hours before and after the interventions. Data were analyzed using one-way ANOVA and the turkey post hoc test at a significant level of 0.05. The results demonstrated 12 weeks of HIIT and MICT significantly increased SOD (P=0.001, P=0.029, respectively) and GPX (P<0.001, P=0.015, respectively). However, significant differences in SOD and GPX (P=0.002, P=0.005, respectively) was only observed between the HIIT and the control group. Additionally, no significant differences was observed between two training groups in SOD and GPX (P>0.05).it seems that HIIT and MICT to enhance the antioxidant system can be considered effective strategies to prevent cancer recurrence in breast cancer survivors.