تأثیر یک دوره تمرینات استقامتی با هدف ممانعت از پیری سلولی بر فعالیت آنزیم تلومراز بافت قلب و لنفوسیت های خون محیطی رت های نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی. پژوهشکده طب ورزشی. پژوهشگاه تربیت بدنی و علوم ورزشی. تهران.ایران

2 استاد فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی، دانشگاه تهران

3 دانشیار گروه فیزیولوژی و علوم ورزشی، دانشگاه تهران، تهران، ایران

چکیده

چکیده
هدف پژوهش حاضر بررسی تأثیر 16 هفته تمرین استقامتی با شدت متوسط بر محتوی آنزیم تلومراز بافت قلب و لنفوسیت‌های خون محیطی رت‌های نر بود. بدین منظور رت‌های تهیه شده از انستیتوی پاستور ایران به صورت تصادفی به دو گروه کنترل (8=n) و ورزشی(8=n) تقسیم شدند. مدت زمان دو هفته برای سازگاری با محیط و تغییر ریتم‌های بیولوژیک (هفته اول) و آشنایی با تردمیل (هفته دوم) در نظر گرفته شد. پس از گذشت دو هفته پروتکل اصلی، شامل یک جلسه فعالیت استقامتی در روز به مدت پنج روز در هفته شروع شد. گروه تجربی به مدت 16 هفته تحت تأثیر تمرینات استقامتی با شدت متوسط قرار گرفتند. در طول هشت هفته اول سرعت تردمیل از 12 متر در دقیقه به 25 متر دقیقه و زمان از 15 دقیقه به 50 دقیقه رسید و این شدت تمرین در طی هشت هفته دوم ثابت نگه داشته شد. پس از اتمام دوره در حالت ناشتا و روز بعد از آخرین جلسه تمرین نمونه‌های مورد نیاز از رت‌ها جمع‌آوری شدند. نتایج نشان داد که تمرینات استقامتی با شدت متوسط موجب افزایش معنادار فعالیت تلومراز در بافت قلبی (0.004=p) و لنفوسیت‌های خون محیطی (0.004=p) شد. به طور کلی می‌توان پیشنهاد داد که فعالیت بدنی منظم با شدت متوسط (65-60%Vo2max ) منجر به فعالسازی آنزیم تلومراز و تثبیت طول تلومر می‌شود و ورزش و فعالیت بدنی از طریق افزایش فعالیت تلومراز در بافت‌های بدن می‌تواند قابلیت زیست سلول، ثبات ژنتیک را بالا ببرد و اثرات ضد پیری خود را بگذارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of a endurance training period with cellular Anti-aging purpose on telomerase enzyme content in cardiac tissue and peripheral blood lymphocytes in rats

نویسندگان [English]

  • Hamze Akbari Boukani 1
  • Ali Asghar Ravasi 2
  • Mohamad Reza Kordi 3
1 Department of exercise physiology, Sport Medicine institute, Sport Science Research Center, Tehran, IRAN
2 Professor, Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran
3 Associate Professor of Physiology and Sport Sciences, University of Tehran, Tehran, Iran
چکیده [English]

Abstract
The purpose of present study was the effect of a endurance training period with cellular Anti-aging purpose on telomerase enzyme content in cardiac tissue and peripheral blood lymphocytes in male rats. For this purpose, rats provided from Pasteur institude of Iran were randomly divided in two group, control (n=8) and exercised groupe (n=8). Two week use for environmental adaptation and biological rhythm (first week) and treadmill familiarity (second week). Then the major protocol of this study began after first two week that include a session endurance exercise per day, five day/week. The intensity of exercise was moderate that exercise group do it for 16 weeks while control group was sedentary in this period. Durig first eight weeks the speed of running on treadmill increased from 15 m/min to 25 m/min and time from 15 min to 25 min progressively but this intensity of exercise fixed during second eight week. One day after end of protocol in fasing situation samoles were collected. The results showed the moderate indurance training significant increased in telomerase content in cardiac tissue (p= 0/004) and peripheral blood lymplocytes (p= 0/004). In summary, the results suggested that the moderate (60-65 % vo2max) and regular physical activity indused activation in telomerase and telomere length stability and exercise and physical activity by telomerase activation in body tissue could increased cellular viability and genomic stability and finally have anti-aging effect.

Key word: Aging, long term exercise, telomere and telomerase

کلیدواژه‌ها [English]

  • aging
  • long term exercise
  • telomere and telomerase

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