تأثیر شش هفته تمرین هوازی و مصرف عصارۀ آبی دانۀ زیرۀ سیاه بر بیان ژن FNDC5 و سطح سرمی آیریزین موش‌های صحرایی نر چاق

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دکتری فیزیولوژی ورزشی و هیأت علمی گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه فنی و حرفه‌ای، تهران، ایران

2 استاد فیزیولوژی ورزشی، گروه علوم ورزشی، دانشگاه بیرجند، ایران

3 استادیار فیزیولوژی پزشکی، گروه فیزیولوژی و فارماکولوژی، دانشگاه علوم پزشکی رفسنجان، ایران

4 استاد فیزیولوژی انسان و پزشکی عمومی، گروه فیزیولوژی، دانشگاه علوم پزشکی لرستان، ایران

چکیده

 
یکی از عوامل مهم در ابتلا به انواع بیماری‌ها، اضافه‌وزن و چاقی است. FNDC5 بیان ژن پروتئین آیریزین در بافت چربی است. هدف از تحقیق حاضر تعیین تأثیر 6 هفته تمرین هوازی و مصرف عصارۀ آبی دانۀ زیرۀ سیاه  بر بیان ژن پروتئین FNDC5، سطح سرمی آیریزین موش‌های صحرایی نر چاق بود. در این تحقیق تجربی، از 24 سر موش صحرایی نر چاق‌شده، به‌دنبال 12 هفته رژیم غذایی پرچرب استفاده شد، که به‌طور تصادفی به چهار گروه 1. کنترل رژیم پرچرب، 2. رژیم پرچرب + تمرین، 3. رژیم پرچرب + عصارۀ زیرۀ سیاه  و 4. گروه رژیم پرچرب + تمرین + عصارۀ زیرۀ سیاه تقسیم شدند. گروه‌های تمرین 5 روز در هفته به مدت 6 هفته تمرین هوازی (تقریباً معادل70٪ - 50% VO2max) انجام دادند. بیان ژن FNDC5، سطح سرمی آیریزین و وزن بدن اندازه‌گیری شد. داده‌ها با استفاده از آنالیز واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنا‌داری 05/0>α تحلیل شدند. نتایج افزایش معنا‌دار بیان ژن FNDC5 و سطح سرمی آیریزین (05/0>P) و کاهش معنا‌دار وزن بدن را در گروه رژیم پرچرب + تمرین + عصارۀ زیره سیاه نسبت به سایر گروه‌ها (05/0>P) و افزایش معنا‌دار بیان ژن FNDC5 گروه تمرین نسبت به گروه کنترل نشان داد (05/0>P). بنابراین، به‌نظر می‌رسد تمرین هوازی با شدت متوسط و مصرف عصارۀ آبی زیرۀ سیاه با افزایش بیان ژن FNDC5 و سطح سرمی آیریزین می‌تواند موجب کاهش وزن و خطرهای ناشی از چاقی شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 6 Weeks of Aerobic Exercise and Aqueous Extract of Caraway Seed on Expression of FNDC5 Gene and Serum Irisin Level in Obese Male Rats

نویسندگان [English]

  • Sedigheh Taherzadeh 1
  • Mehdi Mogharnasi 2
  • Ayat kayedi 3
  • bahram rasoulian 4
1 PhD of Exercise Physiology and Faculty of Department of Physical Education, Faculty of human sciences, Technical and Vocational University (TVU), Tehran, Iran
2 Professor of Exercise Physiology, Department of Sport Sciences, University of Birjand, Birjand, Iran
3 Assistant Professor of Medical Physiology, Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
4 Professor of Human Physiology and General Medicine, Department of Physiology, Lorestan University of Medical Sciences, Lorestan, Iran
چکیده [English]

 
Overweight and obesity are one of the important factors in the prevalence of diseases. FNDC5 is the gene expression of the irisin protein in adipose tissue. The aim of the present study was to determine the effect of 6 weeks of aerobic exercise and aqueous extract of carum carvi on FNDC5 gene expression and serum irisin level in obese male rats. In this experimental study, 24 obese male rats had 12 weeks of high-fat diet and then they were randomly divided into 4 groups: 1. high-fat diet + control 2. high-fat diet + exercise 3. high-fat diet + Carum Carvi extract 4. high-fat diet + exercise + Carum Carvi extract. The exercise groups performed aerobic exercise (approximately 50% - 70% VO2max) 5 days a week for 6 weeks. The gene expression of FNDC5, serum irisin level and body weight were measured. Data were analyzed using one-way ANOVA and Tukey's post hoc tests. The significance level was set at α<0.05. The results showed a significant increase in FNDC5 gene expression, serum level of irisin (P<0.05) and a significant decrease in body weight in the high fat + exercise + Carum Carvi extract group compared to the other groups (P<0.05) and a significant increase in FNDC5 gene expression in the exercise groups compared to the control group (P<0.05). Therefore, it seems that moderate intensity aerobic exercise and aqueous extract of Carum Carvi can decrease body weight and obesity-induced risks by increasing FNDC5 gene expression and serum irisin level.

کلیدواژه‌ها [English]

  • Aerobic exercise
  • Carum Carvi
  • FNDC5 gene
  • Irisin
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