تأثیر دوازده هفته رژیم غذایی پرچرب و تمرین ورزشی با شدت متوسط بر مسیر KLF-15 / KLF-3 / فورین / CTRP-12 بافت چربی احشایی رت‌های نر

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

2 دانشیار فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

3 استاد فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران.

4 دانشیار فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

چکیده

 
    از جمله فاکتورهای مقابله‌کننده با ریسک فاکتورهای متابولیکی، CTRP-12 است. چاقی با تنظیم مثبت فاکتورهای فورین و KLF-3 و تنظیم منفی KLF-15 در مهار CTRP-12 نقش بسزایی دارد. تمرین ورزشی بر پیام‌رسانی سلول‌های چربی تأثیرگذار است. ازاین‌رو هدف مطالعة حاضر بررسی تأثیر 12 هفته تمرین ورزشی با شدت متوسط (MICT) بر CTRP-12، فورین، KLF-3 و KLF-15 بافت چربی احشایی رت‌های نر چاق بود. 15 سر رت نر نژاد ویستار، ابتدا به 2 گروه تغذیه با غذای پرچرب (10 سر) و تغذیه با غذای استاندارد (5 سر) تقسیم شدند. پس از 12 هفته، مجدداً گروه تغذیه با غذای پرچرب به دو گروه MICT و HFD تقسیم شدند. برنامة تمرینی به مدت 12 هفته، 5 جلسه در هفته و با شدت 50-60 درصد حداکثر سرعت اجرا شد. مقادیر متغیرها به روش الایزا اندازه‌گیری شد. تجزیه‌وتحلیل داده‌ها با آزمون آماری ANOVA و نرم‌افزار پریزم 5 انجام گرفت. نتایج مطالعة حاضر نشان داد که HFD سبب افزایش معنا‌دار مقادیر فورین و KLF-3 بافت چربی احشایی شد (05/0P≤). 12 هفته تمرین MICT سبب کاهش معنا‌دار مقادیر فورین، KLF-3 و افزایش معنا‌دار KLF-15 نسبت به گروه HFD شد (05/0P≤). تغییرات CTRP-12 بین گروه‌های مختلف معنا‌دار نبود (386/0F=1.032 , P=). 12 هفته MICT مجزا از تغییرات CTRP-12، در کاهش ریسک فاکتورهای سلولی مضر بافت چربی (فورین و KLF-3) و افزایش فاکتورهای مفید بافت چربی (KLF-15) مؤثر است. ازاین‌رو پیشنهاد می‌شود افراد در معرض چاقی برای سازگاری بهتر سلولی، به تمرینات ورزشی با مدت زمان طولانی‌تر رو بیاورند.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of 12 Weeks of High Fat Diet and Moderate Intensity Training on the KLF-15 / KLF-3 / Furin / CTRP-12 Pathway of Visceral Adipose Tissue in Male Rats

نویسندگان [English]

  • Setareh Amiri 1
  • Asghar Tofighi 2
  • Alireza Shirpoor 3
  • Javad Tolouei Azar 4
1 M.Sc. of exercise physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
2 Associate Professor of exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
3 Professor of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
4 Associate Professor of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
چکیده [English]

 
One of the factors that counteracts with risk factors of metabolic disorder is CTRP-12. Obesity plays an important role in the downregulation of CTRP-12 by positive regulation of furin and KLF-3 factors and negative regulation of KLF-15. Training affects adipocytes signaling. Therefore, the aim of the present study was to investigate the effect of 12 weeks of moderate intensity continuous training (MICT) on the KLF-15 / KLF-3 / furin / CTRP-12 of visceral adipose tissue in male obese rats. 15 male Wistar rats were divided into two groups: high fat diet (n=10) and standard diet (n=5). After 12 weeks, the high fat diet group was again divided into two groups: MICT and HFD. Training program was performed for 12 weeks, 5 sessions per week with intensity of 50-60% of maximum speed. The values of variables were measured by ELISA method. Data were analyzed using ANOVA and Prism 5 software. The results of this study showed that HFD significantly increased the amount of furin and KLF-3 in visceral adipose tissue (P≤0.05). The 12 weeks of MICT significantly decreased the levels of furin, KLF-3 and significantly increased KLF-15 compared with the HFD group (P≤0.05). Changes in CTRP-12 were not significant between groups (F = 1.032, P = 0.386). 12 weeks of MICT, apart from changes in CTRP-12, was effective in decreasing the risk of adipose tissue cellular factors (furin and KLF-3) and in increasing the beneficial factors of adipose tissue (KLF-15). Therefore, it is recommended that those exposed to obesity should get longer training sessions for better cellular adaptation.

کلیدواژه‌ها [English]

  • CTRP-12
  • furin
  • KLF-3
  • KLF-15
  • moderate intensity continuous training

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پژوهش های فیزیولوژی و مدیریت در ورزش
دوره 13، شماره 1 - شماره پیاپی 1
اردیبهشت 1400
صفحه 21-39

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