تغییرات فورین، CTRP-12، TNF-α و پروفایل لیپیدی به هشت هفته تمرین هوازی تناوبی و مقاومتی در زنان دیابتی نوع دو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

2 دانشیار فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

3 استادیار فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران

چکیده

 
استراتژی‌های کنترل فورین، فاکتورهای التهابی و همچنین افزایش مقادیر CTRP-12، در پیشگیری و درمان اختلالات متابولیکی مرتبط با چاقی و دیابت مؤثر است. از جمله استراتژی‌های مؤثر، تمرین ورزشی است. ازاین‌رو هدف پژوهش حاضر بررسی تأثیر 8 هفته تمرین هوازی تناوبی و مقاومتی بر مقادیر فورین، CTRP-12، TNF-α و پروفایل لیپیدی در زنان دیابتی نوع دو بود. 30 زن دیابتی (09/5 ± 9/51 سال)، به‌طور تصادفی به دو گروه تمرین (هوازی تناوبی و مقاومتی) و یک گروه کنترل تقسیم شدند. گروه‌های تمرین به مدت 8 هفته و 3 جلسه در هفته تمرین ورزشی هوازی تناوبی و مقاومتی را انجام دادند. مقادیر فورین، CTRP-12، TNF-α و پروفایل لیپیدی با استفاده از کیت به روش ELISA اندازه‌گیری شد. برای تجزیه‌وتحلیل داده‌ها از آزمون آماری ANCOVA در سطح معناداری 05/0 >P استفاده شد. نتایج آزمون تحلیل کوواریانس نشان داد که در متغیر فورین (046/0 P =، 472/3= F) و CTRP-12 (016/0 P =، 893/4= F) بین گروه‌های پژوهش پس از 8 هفته مداخله تفاوت معنا‌داری وجود داشت. نتایج آزمون تعقیبی LSD نشان داد، تمرین مقاومتی نسبت به گروه کنترل موجب کاهش معنا‌دار فورین (020/0 P =) و افزایش معنا‌دار CTRP-12 (004/0 P =) شد. این در حالی بود که معنا‌داری TNF-a تأیید نشد (055/0 P =، 245/3= F). به‌نظر می‌رسد تغییرات CTRP-12 با تمرین مقاومتی در نمونۀ دیابتی به مقدار بیشتر تحت تأثیر فورین و به مقدار کمتر تحت تأثیر آدیپوکاین التهابی TNF-α قرار می‌گیرد، که این مسیر مقاومت به انسولین را نیز کنترل می‌کند.

کلیدواژه‌ها

عنوان مقاله [English]

Furin, CTRP-12, TNF-α and Lipid Profile Changes during 8 Weeks of Aerobic Interval and Resistance Training in Women with Type 2 Diabetes

نویسندگان [English]

  • Ebrahim Froozandeh 1
  • Asghar Tofighi 2
  • Javad Tolouei azar 3
1 PhD Student of Exercise Physiology, Urmia University
2 Associate Professor of Exercise Physiology, Urmia University
3 Assistant Professor of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran.
چکیده [English]

Strategies of furin control, inflammatory factors and also increased CTRP-12 are effective to prevent and treat metabolic disorders associated with obesity and diabetes. One of the effective strategies is training. Therefore, the aim of this study was to investigate the effect of 8 weeks of aerobic interval and resistance training on levels of furin, CTRP-12, TNF-α and lipid profile in type 2 diabetic women. 30 diabetic women (51.9 ± 5.09 years) were randomly divided into two groups of training (aerobic interval and resistance) and one control group. The training groups performed aerobic interval and resistance training for 8 weeks and 3 sessions per week. Furin, CTRP-12, TNF-α, and lipid profile were measured using ELISA kit.  Data were analyzed by ANCOVA at the significance level (P<0.05). The results of ANCOVA showed a significant difference between the study groups after 8 weeks of intervention in the furin (P=0.046, F=3.472) and CTRP-12 (P=0.016, F=4.893). The results of LSD post hoc test showed that resistance training significantly decreased furin (P=0.020) and CTRP-12 (P=0.004) compared with the control group. However, the significance of TNF-a was not confirmed (P=0.055, F=3.245). It seems that changes of CTRP-12 with resistance training are more affected by furin and less affected by TNF-α inflammatory adipokine in diabetic patients. This pathway controls insulin resistance as well.

کلیدواژه‌ها [English]

  • aerobic interval training
  • CTRP-12
  • diabetic women
  • furin
  • resistance training
  • TNF-α

مراجع

1. Association AD. Diagnosis and classification of diabetes mellitus. Diabetes care. 2014;37(Supplement 1):S81-S90.
2. Hossain P, Kawar B, El Nahas M. Obesity and diabetes in the developing world—a growing challenge. 2009.
3. Rockette-Wagner B, Edelstein S, Venditti EM, Reddy D, Bray GA, Carrion-Petersen ML, et al. The impact of lifestyle intervention on sedentary time in individuals at high risk of diabetes. Diabetologia. 2015;58(6):1198-202.
4. Cho N, Shaw J, Karuranga S, Huang Y, da Rocha Fernandes J, Ohlrogge A, et al. IDF Diabetes Atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045. Diabetes research and clinical practice. 2018;138:271-81.
5. Saed L, Deihim Z, Naghshbandi MK, Rajabnia M, Naleini SN. Cardiovascular events in patients with over 10 years history of type 2 diabetes mellitus. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 2019;13(1):68-72.
6. Achari A, Jain S. Adiponectin, a therapeutic target for obesity, diabetes, and endothelial dysfunction. International journal of molecular sciences. 2017;18(6):1321.
7. Hofmann C, Chen N, Obermeier F, Paul G, Büchler C, Kopp A, et al. C1q/TNF-related protein-3 (CTRP-3) is secreted by visceral adipose tissue and exerts antiinflammatory and antifibrotic effects in primary human colonic fibroblasts. Inflammatory bowel diseases. 2011;17(12):2462-71.
8. Enomoto T, Ohashi K, Shibata R, Higuchi A, Maruyama S, Izumiya Y, et al. Adipolin/C1qdc2/CTRP12 protein functions as an adipokine that improves glucose metabolism. Journal of Biological Chemistry. 2011;286(40):34552-8.
9. Tan BK, Chen J, Adya R, Ramanjaneya M, Patel V, Randeva HS. Metformin increases the novel adipokine adipolin/CTRP12: role of the AMPK pathway. Journal of Endocrinology. 2013;219(2):101-8.
10. Ohashi K, Shibata R, Murohara T, Ouchi N. Role of anti-inflammatory adipokines in obesity-related diseases. Trends in Endocrinology & Metabolism. 2014;25(7):348-55.
11. Wei Z, Peterson JM, Lei X, Cebotaru L, Wolfgang MJ, Baldeviano GC, et al. C1q/TNF-related protein-12 (CTRP12), a novel adipokine that improves insulin sensitivity and glycemic control in mouse models of obesity and diabetes. Journal of Biological Chemistry. 2012;287(13):10301-15.
12. Wei Z, Lei X, Seldin MM, Wong GW. Endopeptidase cleavage generates a functionally distinct isoform of C1q/tumor necrosis factor-related protein-12 (CTRP12) with an altered oligomeric state and signaling specificity. Journal of Biological Chemistry. 2012;287(43):35804-14.
13. Tellier E, Nègre-Salvayre A, Bocquet B, Itohara S, Hannun YA, Salvayre R, et al. Role for furin in tumor necrosis factor alpha-induced activation of the matrix metalloproteinase/sphingolipid mitogenic pathway. Molecular and cellular biology. 2007;27(8):2997-3007.
14. Enomoto T, Shibata R, Ohashi K, Kambara T, Kataoka Y, Uemura Y, et al. Regulation of adipolin/CTRP12 cleavage by obesity. Biochemical and biophysical research communications. 2012;428(1):155-9.
15. Gordon B, Chen S, Durstine JL. The effects of exercise training on the traditional lipid profile and beyond. Translational Journal of the American College of Sports Medicine. 2016;1(18):159-64.
16. Lakka TA, Laaksonen DE. Physical activity in prevention and treatment of the metabolic syndrome. Applied physiology, nutrition, and metabolism. 2007;32(1):76-88.
17. Dumortier M, Brandou F, Perez-Martin A, Fedou C, Mercier J, Brun J. Low intensity endurance exercise targeted for lipid oxidation improves body composition and insulin sensitivity in patients with the metabolic syndrome. Diabetes & metabolism. 2003;29(5):509-18.
18. Trapp EG, Chisholm DJ, Freund J, Boutcher SH. The effects of high-intensity intermittent exercise training on fat loss and fasting insulin levels of young women. International journal of obesity. 2008;32(4):684.
19. Patel SJ, Hanks LJ, Ashraf AP, Gutierrez OM, Bamman MM, Casazza K. Effects of 8 week resistance training on lipid profile and insulin levels in overweight/obese peri-pubertal boys-a pilot study. Journal of Diabetes Research and Clinical Metabolism. 2015; 4(1).2
20. Rezaian N, Ravasi AA, Sori R and et al. Effect of Resistance Training on Serum Levels of Adipolin
and Insulin Resistance in Obese Women. Science Of Biology Journal. 2015.
21. Rahmatollahi M, Ravasi A, Soori R. Effect of 8 Weeks of Low-Intensity Continuous Training on Plasma Adipolin, Insulin Resistance, and Weight of Fatty Fat-Filled Rats. Adv Obes Weight Manag Control. 2017;7(5):00211.
22. Keating S, Johnson N, Mielke G, Coombes J. A systematic review and meta‐analysis of interval training versus moderate‐intensity continuous training on body adiposity. Obesity reviews. 2017;18(8):943-64.
23. AghaAlinejad H, Mehrabani J, AnsariDogahe R, Piri M. The influence of resistance, endurance, and combined resistance-endurance exercise training on interleukin-18 and C-reactive protein level in inactive female adolescents. Tabari Journal Of Preventive Medicine. 2016;2(1):38-47.
24. Pittas AG, Joseph NA, Greenberg AS. Adipocytokines and insulin resistance. The Journal of Clinical Endocrinology & Metabolism. 2004;89(2):447-52.
25. Tan BK, Chen J, Hu J, Amar O, Mattu HS, Ramanjaneya M, et al. Circulatory changes of the novel adipokine adipolin/CTRP 12 in response to metformin treatment and an oral glucose challenge in humans. Clinical endocrinology. 2014;81(6):841-6.
26. Soori R, Asad M, Barahouei-Jamar Z, Rezaeian N. The effect of aerobic training on the serum level of adipolin and insulin resistance in overweight men. Feyz Journal of Kashan University of Medical Sciences. 2016;19.
27RAHMATOLLAHI M, RAVASI A, SOORI R, ONEGH B. Adipolin and Insulin Resistance Response to Two Types of Exercise Training in Type 2 Diabetic Male Rats. 2017.
28. Enomoto T, Ohashi K, Shibata R, Kambara T, Uemura Y, Yuasa D, et al. Transcriptional regulation of an insulin-sensitizing adipokine adipolin/CTRP12 in adipocytes by Krüppel-like factor 15. PloS one. 2013;8(12):e83183.
29. Sigal RJ, Kenny GP, Wasserman DH, Castaneda-Sceppa C, White RD. Physical activity/exercise and type 2 diabetes: a consensus statement from the American Diabetes Association. Diabetes care. 2006;29(6):1433-8.
30. Wärnberg J, Cunningham K, Romeo J, Marcos A. Physical activity, exercise and low-grade systemic inflammation. Proceedings of the Nutrition Society. 2010;69(3):400-6.
31. Mokhtarzade M, Ranjbar R, Majdinasab N, Patel D, Shamsi MM. Effect of aerobic interval training on serum IL-10, TNFα, and adipokines levels in women with multiple sclerosis: possible relations with fatigue and quality of life. Endocrine. 2017;57(2):262-71.
 
پژوهش های فیزیولوژی و مدیریت در ورزش
دوره 12، شماره 3
آذر 1399
صفحه 25-40

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