تأثیر تمرینات تناوبی شدید بر بیان ژن‌های کمرین و آدیپسین در بافت کبد مدل حیوانی استئاتوزیس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد فیزیولوژی ورزشی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران.

2 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

برخی آدیپوکین‌ها در پاتوژنز بیماری کبد چرب غیرالکلی نقش دارند. آدیپوکین‌ها در بسیاری از فرایندهای متابولیک نقش دارند. هدف از این پژوهش، بررسی تأثیر تمرینات تناوبی شدید بر بیان ژن‌های کمرین و آدیپسین در بافت کبد مدل حیوانی استئاتوزیس بود. در این تحقیق تجربی، 32 سر رت نر نژاد ویستار با وزن 200-250 گرم انتخاب شدند و به‌طور تصادفی در چهار گروه شامل کنترل سالم، کبد چرب، HIIT و کبد چرب+ HIIT قرار گرفتند. رت‌ها با تجویز داروی تتراسایکلین خوراکی با دوز 140میلی‌گرم به ازای هر کیلوگرم از وزن بدن (به‌صورت محلول در 2 میلی‌لیتر آب) به مدت هفت روز مبتلا به کبد چرب شدند. پروتکل HIIT شامل پنج تناوب دودقیقه‌ای دویدن با 85 تا 90 درصد VO2max و به‌دنبال آن 1 دقیقه دویدن در 30-40 درصد VO2max  بین هر وهله، پنج جلسه در هفته و به مدت 5 هفته روی نوار گردان اجرا شد. میزان بیان ژن کمرین و آدیپسین در بافت کبد به روش Real Time PCR اندازه‌گیری شد. داده‌ها با استفاده از آزمون تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در سطح معنا‌داری 05/0 P< تجزیه‌وتحلیل شد. نتایج نشان داد بیان ژن کمرین و آدیپسین بافت کبد در گروه کبد چرب نسبت به گروه کنترل سالم به‌طور معنا‌داری بیشتر بود (001/0P=). بیان ژن کمرین و آدیپسین بافت کبد در گروه HIIT و کبد چرب+ HIIT نسبت به گروه کبد چرب به‌طور معنا‌داری کمتر بود (001/0P=). به‌نظر می‌رسد برنامة تمرین HIIT می‌تواند به بهبود سطوح آدیپوکاین‌ها در بیماری کبد چرب کمک کند، ازاین‌رو این روش تمرینی به‌منظور مزایای آن در بیماری کبد چرب پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of High-intensity Interval Training on Chemrine and Adipsin Gene Expression of liver Tissue in Animal of Steatosis

نویسندگان [English]

  • Samira Najafabadi 1
  • Saeedeh Shadmehri 2
1 M.Sc. Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran,
2 Assistant Professor, Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran.
چکیده [English]

Some adipokines are involved in the pathogenesis of nonalcoholic fatty liver disease. The aim of this study was to investigate the effect of high-intensity interval training on Chemrine and Adipsin gene expression of liver tissue in animal of steatosis. In this experimental study, 32 rats (weighing 200-250 gr) were selected and randomly divided into 4 groups including healthy control, fatty liver, HIIT and fatty liver + HIIT group. Rats were infected with fatty liver by oral tetracycline at a dose of 140 mg/kg (soluble in 2 ml of water) for 7 days. HIIT protocol contained 5 intervals 2 min running session at 85–90% of VO2max followed by 1 min running at 30–40% of VO2max between each session, five sessions per week for 5 weeks. The Chemrine and Adipsin gene expression in the liver tissue samples was measured by Real Time PCR. Data were analyzed by One-way ANOVA and Tukey post hoc tests at the P <0.05. The results showed that the Chemrine and Adipsin gene expression of liver tissue was significantly higher in fatty liver group than the healthy control group (P=0.001). Chemrine and Adipsin gene expression in liver tissue was significantly lower in HIIT and fatty liver + HIIT group’s than the fatty liver group (P=0.001). It seems that the HIIT training program can help improve the levels of adipokines in fatty liver disease, so this training method is recommended for its benefits in fatty liver disease.

کلیدواژه‌ها [English]

  • Adipsin
  • Chemrine
  • Fatty liver
  • High-Intensity Interval Training
  • Rats

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